Journal: Journal of Virology
Article Title: Cytoplasmic translocation of tripartite motif-containing 28 is critical for PRRSV-induced autophagy through promoting Vps34-Beclin1 complex formation
doi: 10.1128/jvi.01133-25
Figure Lengend Snippet: PRRSV-induced nuclear export of TRIM28 depends on CRM1. ( A ) Immunoblot analysis of TRIM28 in cytoplasmic and nuclear fractions of Marc-145 cells infected with HP-PRRSV (MOI=0.1) for 24 h, or treated with 15 nM LMB for 2 h before infection. ( B ) Confocal analysis of Marc-145 cells transfected with GFP-TRIM28 plasmid, infected with HP-PRRSV (MOI=0.1) for 24 h, or treated with 15 nM LMB for 2 h before infection, 24 h post-transfection. scale bars, 10 µm. ( C ) Immunoblot analysis of Flag-CRM1 and TRIM28 in immunoprecipitated and whole-cell lysates of HEK-293T cells. ( D ) Immunoblot analysis of Flag-CRM1 and TRIM28 in immunoprecipitated and whole-cell lysates of Marc-145 cells infected with HP-PRRSV (MOI=0.1) or untreated for 24 h. ( E ) Immunoblot analysis of Flag-CRM1 and TRIM28 in immunoprecipitated and whole-cell lysates of Marc-145 cells infected with HP-PRRSV (MOI=0.1) for 24 h, or treated with 15 nM LMB for 2 h before infection. ( F ) Immunoblot analysis of Flag-CRM1, GFP-TRIM28, and HA-Nsp4 in immunoprecipitated and whole-cell lysates of HEK-293T cells. ( G ) Schematic representation of TRIM28 (WT) and its truncation mutants (left), and co-immunoprecipitation (Co-IP) analysis of CRM1 interaction with Flag-TRIM28 (WT) and TRIM28 truncation mutants in HEK-293T cells (right).
Article Snippet: Anti-CRM1 mouse MAb , Proteintech , 66763-1-lg.
Techniques: Western Blot, Infection, Transfection, Plasmid Preparation, Immunoprecipitation, Co-Immunoprecipitation Assay
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